Novel Next Generation Sequencing Library Prep kits for
- Liquid biopsy analysis cell free RNA and DNA (cfRNA/cfDNA) from serum, exosome, and culture media
- Metagenomics for pathogen (bacterial, fungal, and/or viral total nucleic acid)
- Epigenetic and gene expression analysis from cell lysate with a single reaction
- Total RNA analysis without rRNA removal
- Single cell WGA without DOP-PCR or MDA
- 3D structure of chromosomes at single cell and single base resolution
ted-Meta™ Library Prep Kit closes the gap between clinical sample and metagenomics analysis. In a single reaction, extracted total nucleic acid (1 – 100ng) or cell lysates (1000 – 10K cells) can turn into a fragment library ready for sequencing on Illumina® platform in ~2hrs. Pre- and/or post-library prep enrichment can further condense the final readouts and hence increase cost effectiveness.
Extracted nucleic acid from clinical specimen
TR-1016-08 (8 rxn)
TR-1016-96 (96 rxn)
The ted-CapALL™ kit converts DNA and RNA molecules simultaneously into next generation sequencing (NGS) libraries. The robustness of the ted-CapALL™ workflow allows the analysis of pg amounts of nucleic acids directly from limited samples (5pg – 100ng) or cell lysates (1 – 10k cells). The concurrent analysis of DNA and RNA allows cell type identification by linking gene expression data to chromatin accessibility profiles. The ted-CapALL™ Kit generates sequencing libraries for Illumina® sequencing platforms.
Liquid biopsy analysis cell free RNA and DNA
TR-1012-08 (8 rxn)
TR-1012-96 (96 rxn)
The ted-mRNA™ kit enables full-length analysis of mRNA (5pg – 5ng) ideal for transcript analysis and gene counting. In 2 hours the simple one-tube workflow converts poly(A)-enriched RNA into an RNA-Seq library. The ted-mRNA™ kit generates sequencing libraries for Illumina® sequencing platforms.
TR-1015-08 (8 rxn)
TR-1015-96 (96 rxn)
The ted-RNA™ 5’ kit enables strand-specific analysis of total RNA (1-200ng) or mRNA (100pg – 100ng) ideal for transcript analysis and gene counting. Without ribosomal RNA removal, the simple 2 hour one-tube workflow creates stranded RNA-Seq libraries covering . The ted-RNA™ 5’ kit generates sequencing libraries for Illumina® sequencing platforms.
Extracted total RNA
TR-1021-08 (8 rxn)
TR-1021-96 (96 rxn)
The ted-RNA™ 3’ kit enables strand-specific analysis of mRNA (1-250ng). The simple 2 hour one-tube workflow creates RNA-Seq libraries starting with total RNA or poly(A)-enriched mRNA. The ted-RNA™ 3’ kit applies a simple and fast protocol to initiate reverse transcription from the poly(A) tail. No ribosomal RNA removal required; data sets contain less than 0.1% rRNA reads.
Extracted total RNA
TR-1031-08 (8 rxn)
TR-1031-96 (96 rxn)
1 to 10 cells
>2pg gDNA >5kb
TD-1011-08 (8 rxn)
TD-1011-96 (96 rxn)
Kit includes reagents for a novel whole genome amplification (WGA) and conversion of a genome of a single cell into Illumina® sequencing libraries. Provides micro-CNV detection with kilobase resolution and ~ 85% coverage of the genome from 1 cell.
Sorted single cell(s)
TD-1001-08 (8 rxn)
TD-1001-96 (96 rxn)
This Indexing PCR Kit supplement both ted-RNA™ and ted-DNA™ kits with a high-fidelity PCR mastermix and 16 different indexes combination.
Non-indexed X Gen’s RNA/DNA libraries
Accurate SNV detection in single cells by whole-genome amplification of complementary strands. A new single-cell WGA method termed multiplexed end-tagging amplification of complementary strands (ted-scDNA™ Stranded), which significantly reduced FPs by virtue of DNA complementarity.
Sorted single Cell
Sorted single cell