Novel Next Generation Sequencing Library Prep kits for
- Liquid biopsy analysis cell free RNA and DNA (cfRNA/cfDNA) from serum, exosome, and culture media
- Metagenomics for pathogen (bacterial, fungal, and/or viral total nucleic acid)
- Epigenetic and gene expression analysis from cell lysate with a single reaction
- Total RNA analysis without rRNA removal
- Single cell WGA without DOP-PCR or MDA
- 3D structure of chromosomes at single cell and single base resolution
Kit Name
Description
Sample Input
Catalog No
Price (US$)
ted-Meta™ Library Prep Kit closes the gap between clinical sample and metagenomics analysis. In a single reaction, extracted total nucleic acid (1 – 100ng) or cell lysates (1000 – 10K cells) can turn into a fragment library ready for sequencing on Illumina® platform in ~2hrs. Pre- and/or post-library prep enrichment can further condense the final readouts and hence increase cost effectiveness.
Cell lysate
Extracted nucleic acid from clinical specimen
TR-1016-08 (8 rxn)
TR-1016-96 (96 rxn)
$400
$3,840
The ted-CapALL™ kit converts DNA and RNA molecules simultaneously into next generation sequencing (NGS) libraries. The robustness of the ted-CapALL™ workflow allows the analysis of pg amounts of nucleic acids directly from limited samples (5pg – 100ng) or cell lysates (1 – 10k cells). The concurrent analysis of DNA and RNA allows cell type identification by linking gene expression data to chromatin accessibility profiles. The ted-CapALL™ Kit generates sequencing libraries for Illumina® sequencing platforms.
Liquid biopsy analysis cell free RNA and DNA
Cell lysate
Exosome
Pathogen (bacterial/viral)
TR-1012-08 (8 rxn)
TR-1012-96 (96 rxn)
$350
$3,360
The ted-mRNA™ kit enables full-length analysis of mRNA (5pg – 5ng) ideal for transcript analysis and gene counting. In 2 hours the simple one-tube workflow converts poly(A)-enriched RNA into an RNA-Seq library. The ted-mRNA™ kit generates sequencing libraries for Illumina® sequencing platforms.
poly(A)-enriched RNA
Total RNA
TR-1015-08 (8 rxn)
TR-1015-96 (96 rxn)
$350
$3,360
The ted-RNA™ 5’ kit enables strand-specific analysis of total RNA (1-200ng) or mRNA (100pg – 100ng) ideal for transcript analysis and gene counting. Without ribosomal RNA removal, the simple 2 hour one-tube workflow creates stranded RNA-Seq libraries covering . The ted-RNA™ 5’ kit generates sequencing libraries for Illumina® sequencing platforms.
Extracted total RNA
poly(A)-enriched RNA
TR-1021-08 (8 rxn)
TR-1021-96 (96 rxn)
$400
$3,840
The ted-RNA™ 3’ kit enables strand-specific analysis of mRNA (1-250ng). The simple 2 hour one-tube workflow creates RNA-Seq libraries starting with total RNA or poly(A)-enriched mRNA. The ted-RNA™ 3’ kit applies a simple and fast protocol to initiate reverse transcription from the poly(A) tail. No ribosomal RNA removal required; data sets contain less than 0.1% rRNA reads.
Extracted total RNA
poly(A)-enriched RNA
TR-1031-08 (8 rxn)
TR-1031-96 (96 rxn)
$300
$2,880
2.5 hrs WGA protocol to fragment, amplify, and archive ultra low amount of DNA, e.g. single cell genome.
1 to 10 cells
>2pg gDNA >5kb
TD-1011-08 (8 rxn)
TD-1011-96 (96 rxn)
$250
$2400
Kit includes reagents for a novel whole genome amplification (WGA) and conversion of a genome of a single cell into Illumina® sequencing libraries. Provides micro-CNV detection with kilobase resolution and ~ 85% coverage of the genome from 1 cell.
Sorted single cell(s)
TD-1001-08 (8 rxn)
TD-1001-96 (96 rxn)
$400
$3840
This Indexing PCR Kit supplement both ted-RNA™ and ted-DNA™ kits with a high-fidelity PCR mastermix and 16 different indexes combination.
Non-indexed X Gen’s RNA/DNA libraries
PI-0001
$120
Accurate SNV detection in single cells by whole-genome amplification of complementary strands. A new single-cell WGA method termed multiplexed end-tagging amplification of complementary strands (ted-scDNA™ Stranded), which significantly reduced FPs by virtue of DNA complementarity.
Sorted single Cell
3D structure of chromosomes at single cell and single base resolution. Detailed SOP see link literatures.
Sorted single cell