ted-RNA™ 3’ WTA Library Prep Kit

$300.00$2,880.00

Gene expression profiles from a single-reaction
An improved version of MALBAC-DT that converts mRNA into Illumina® compatible mRNA-seq libraries by extension from the Poly(A) tail with a simple rapid protocol. Less than 0.1% rRNA. 1 ng to 100 ng mRNA input. For efficient gene expression analysis.
User needs to provide PCR and Illumina® indexing reagents.

[LINK] Publication of MALBAC-DT

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Description

For Illumina® sequencing systems

ted-RNA 3′ WTA Library Prep Kit utilizes “ted” technology to enable the simplest workflow to obtain basic information, e.g. CNV, gene count, and gene expression, etc from as low as 1ng total RNA input. This protocol does not require ribosomal RNA removal as rRNA is converted into library molecules at substantially low efficiency.

Cat. No. Format Storage Kit stability
TR-1031-08 8 reactions per kit -20°C 1 year from the date of manufacture
TR-1031-96 96 reactions per kit -20°C 1 year from the date of manufacture

Attributes

Input sample Total RNA or mRNA
Input amount 1 - 250 ng of total RNA
Data produced Sequencing reads for 3’-region of PolyA mRNA.
Data processing Trim first 20-base UMI N16+V4 from Read 1
seqtk trimfq -b 20 $Read1 ; with seqtk from https://github.com/lh3/seqtk
Protocol time
  1. RNA to library: 30 minutes (1 tube reaction)
  2. Indexing PCR & QC: 1 hr.
RNA gene coverage > 90% of mRNA genes detected.
Ribosomal RNA carryover < 1% without ribosomal removal from total RNA
Reaction volume 10 uL final volume per reaction
Kit components
  1. ted-RNA 3’ enzyme mix: A proprietary composition of novel enzymes that convert PolyA mRNA into an Illumina compatible sequencing library, in a single tube reaction.
  2. ted-RNA 3’ primer mix: Primers designed to convert PolyA mRNA into an NGS library.
  3. Preamp Oligos
User provided components
  • total RNA
  • SPRI beads system, e.g. AMPure XP or Kapa Pure Bead, etc
  • PCR reagents and indexing adapters (our Indexing PCR Kit (cat. no. PI-0001)is sold separately and compatible with Nextera kits.)
Applications Highly efficient gene expression analysis of PolyA mRNA with minimal sequencing depth.

Downloads

Reproducibility & sensitivity

Mapping distribution (STAR alignment)

Transcript coverage

Gene count of 1 & 10 ng total RNA input at TPM>1

Library structure

X Gen US CO’s products are for Research Use Only. They cannot be used for diagnostic or therapeutic applications. These products cannot be resold, modified for resale, used in the manufacturing of commercial products or transferred to third parties without prior written approval from X Gen US CO. Prior written approval is required for service providers who incorporate X Gen US CO’s products into their service offerings. Prior written approval is required for integrating X Gen US CO’s products into instrumentation systems (automation, liquid handling, cell sorting and related).

X Gen US CO, the logo, ted-RNA, ted-DNA, CapALL and ted-technology are trademarks of X Gen US CO. Illumina is a registered trademark of Illumina, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.

Additional information

Reactions per kit

8, 96