ted-mRNA™ Library Prep Kit

$350.00$3,360.00

RNA profiles from a single reaction
Determines gene expression profiles directly from purified mRNA in a single tube reaction.
User needs to provide PCR and Illumina® indexing reagents.

Cat. No. : N/A Category:
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Description

For Illumina sequencing systems

Cat. No. Format Storage Kit stability
TR-1015-08 8 reactions per kit -20°C 1 year from the date of manufacture
TR-1015-96 96 reactions per kit -20°C 1 year from the date of manufacture

The ted-mRNA™ kit generates sequencing libraries for Illumina® sequencing platforms. The simple one-tube reaction enables library preparation of full length transcripts in 2.5 hours from RNA sample to sequencing. This protocol requires poly(A) enriched RNA input.

Attributes:

Protocol [PDF] ted-mRNA™ Library Prep Kit User Guide
Input sample 5 pg to 5 ng mRNA
Data produced Sequencing reads for coding RNA with 3' to 5' coverage.
Protocol time
  1. cDNA library preparation 40min
  2. Preamplification 40min
  3. Indexing PCR & QC 40 min
Transcripts coverage Full length transcript coverage.
Gene Counts > 14K genes detected TPM>1 from 1ng mRNA (extracted from UHRR).
Reaction volume 10 uL final volume per reaction
Kit components
  1. ted-mRNA™ Enzyme mix: A proprietary composition of novel enzymes that convert RNA into an Illumina compatible sequencing library, in a single tube reaction
  2. ted-mRNA™ Primer mix: Primers designed to convert RNA and chromatin into an NGS library.
  3. Preamp Oligos
User provided components
  • poly(A) enriched RNA, purified mRNA
  • SPRI beads system, e.g. AMPure XP or Kapa Pure Bead, etc
  • PCR reagents and indexing adapters (our PCR kit is sold separately)
Applications Simultaneous analysis of
  • mRNA, non-polyA coding RNA, non-coding RNA (nc and lncRNA) expression
  • Protocol is compatible with samples from prokaroytic and eukayotic organisms.

Product Features:

Reproducibility & sensitivity
Mapping statistics
Genes detected
Transcripts coverage
Library structure

XGen products are for Research Use Only. They cannot be used for diagnostic or therapeutic applications. XGen products cannot be resold, modified for resale, used in the manufacturing of commercial products or transferred to third parties without prior written approval from XGen. Prior written approval is required for service providers who incorporate XGen products into their service offerings. Prior written approval is required for integrating XGen products into instrumentation systems (automation, liquid handling, cell sorting and related).

XGen, the XGen logo, ted-RNA, ted-DNA, CaptureAll and ted-technology are trademarks of Xgen Laboratories, Inc. Illumina is a registered trademark of Illumina, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.

Additional information

Reactions per kit

8, 96