ted-scDNA(™) Library Preparation Kit

$400.00$3,840.00

85% human genome coverage from a single cell at 30x sequencing depth
ted-scDNA™ kit includes reagents for a novel whole genome amplification and conversion of a genome of a single to few cells into Illumina® sequencing libraries. Provides micro-CNV detection with kilobase resolution across the genome and ~ 85% coverage of the genome from 1 cell.
User needs to provide PCR and Illumina® indexing reagents.

Cat. No. : N/A Category:
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Description

For Illumina sequencing systems

Cat. No. Format Storage Kit stability
TD-1001-08 8 reactions per kit -20°C 1 year from the date of manufacture
TD-1001-96 96 reactions per kit -20°C 1 year from the date of manufacture

The ted-scDNA kit generates sequencing libraries for Illumina® sequencing platforms. The novel “ted” technology minimizes number of steps to prepare a library ready for sequencing in ~4hrs.

Attributes:

Protocol [PDF] ted-scDNA™ Library Preparation Kit User Guide
Input sample 1-10 cell.
Input amount Sorted single cell in cell lysis buffer.
Data produced Sequencing reads for whole genome sequencing of individual single cells.
Protocol time From single cell to library ~ 4 hr
Genome coverage >85% of the genome covered at 30x sequencing depth
Reaction volume 6 uL final volume per reaction
Kit components ted-DNA WGA enzyme mix 1&2: A proprietary composition of novel enzymes that convert chromatin from single cell lysate into an Illumina® compatible whole genome sequencing library, in a single tube reaction.
User provided components
  • Single to ~10 whole cells or lysate
  • SPRI beads system, e.g. AMPure XP or Kapa Pure Bead, etc
  • PCR reagents and indexing adapters (our PCR kit is sold separately)
Applications Whole genome sequencing of single cells for aneuploidies, large structural variation at sequencing depths of < 1x to detection of micro-CNV at sequencing depths of 30x.

Performance data (click the thumbnail to enlarge image):

Sequencing coverage
Coverage comparison to existing technology
(ted-scDNA is an improved version of DIANTI)
85% coverage of a single cell
Single cell coverage

XGen products are for Research Use Only. They cannot be used for diagnostic or therapeutic applications. XGen products cannot be resold, modified for resale, used in the manufacturing of commercial products or transferred to third parties without prior written approval from XGen. Prior written approval is required for service providers who incorporate XGen products into their service offerings. Prior written approval is required for integrating XGen products into instrumentation systems (automation, liquid handling, cell sorting and related).

XGen, the XGen logo, ted-RNA, ted-DNA, CaptureAll and ted-technology are trademarks of Xgen Laboratories, Inc. Illumina is a registered trademark of Illumina, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.

Additional information

Reactions per kit

8, 96