$350.00 – $3,360.00
The ted-CapALL™ kit converts DNA and RNA molecules simultaneously into next generation sequencing (NGS) libraries. The robustness of the ted-CapALL™ workflow allows the analysis of picograms of nucleic acids directly from limited samples (5pg – 100ng) or cell lysates (> 10 cells). The concurrent analysis of DNA and RNA allows cell type identification by linking gene expression data to chromatin accessibility profiles. The ted-CapALL™ Kit generates sequencing libraries for Illumina® sequencing platforms.
For Illumina sequencing systems
|Cat. No.||Format||Storage||Kit stability|
|TR-1012-08||8 reactions per kit||-20°C||1 year from the date of manufacture|
|TR-1012-96||96 reactions per kit||-20°C||1 year from the date of manufacture|
The ted-CapALL™ Kits generate sequencing libraries for Illumina® sequencing platforms. The novel “ted” technology used in these kits generate gene expression data as well as nucleosomal coverage data directly from cell and tissue homogenates providing orthogonal data to correlate gene expression with nucleosomal epigenetic profiles. ted-CapALL™ protocols do not require ribosomal RNA removal as rRNA is converted into library molecules at substantially low efficiency.
|Input sample||1 - 10000 cells lysate
5 pg - 100 ng total RNA or mRNA
Total nucleic acid in medium or serum
|Input amount|| 7 uL of extracted RNA
6 uL of lysates with 1 to 10000 cells
2 uL of cell medium or serum
|Data produced||Sequencing reads delineating nucleosomal-free regions of the genome.
Sequencing reads for coding and non-coding RNA with < 10% of total reads mapping to ribosomal RNA.
|Data processing||Q20 filter, trim adaptors, remove <20bp reads and duplicates
seqtk trimfq -q 0.01 $Read2 ; with bioinformatic tool seqtk from https://github.com/lh3/seqtk.
|Chromatin coverage||Nucleosomal-free regions mapped to > 80% of the genome.|
|RNA gene coverage||> 80% of genes detected as compared to the ted-RNA™ kit for the same sample.|
|Transcript coverage||> 80% of bases from 5’ to 3’ … for mRNA and ncRNA|
|Ribosomal RNA carryover||< 10% without ribosomal removal from total RNA|
|Reaction volume||10 uL final volume per reaction|
|User provided components||
|Applications||Simultaneous analysis of
Concordance between total RNA and mRNA input
Gene counts of different amount of input
Reproducibility between different input amounts (5 to 500 picograms)
Gene coverage 5′ to 3′
Mapped reads categories
X Gen US CO’s products are for Research Use Only. They cannot be used for diagnostic or therapeutic applications. These products cannot be resold, modified for resale, used in the manufacturing of commercial products or transferred to third parties without prior written approval from X Gen US CO. Prior written approval is required for service providers who incorporate X Gen US CO’s products into their service offerings. Prior written approval is required for integrating X Gen US CO’s products into instrumentation systems (automation, liquid handling, cell sorting and related).
X Gen US CO, the logo, ted-RNA, ted-DNA, CapALL and ted-technology are trademarks of X Gen US CO. Illumina is a registered trademark of Illumina, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.
|Reactions per kit||